The generation of cell lines overexpressing particular proteins on which drug discovery programs are often based, is necessary for assay development in screening campaigns using initial target-based identification of hit compounds. Recombinant stable cell lines are also used for toxicity studies and general in vitro testing.
Methods to be used and the cell background for the generation of a cell line could depend on several factors: i) the complexity of protein (e.g. dimensions, carrying antibiotic resistance, presence of tags etc.)…Find out more
Proteins typically undergo a proper folding via intracellular chaperone machinery. Folding confers to proteins the spatial characteristics that define their role in the cells, however the folding process might not always work properly. For intrinsic changes of protein characteristics (e.g. mutations and/or improper elongation, PTMs etc.) or for defective chaperone activity, proteins can assume a wrong conformation. In this case complex intracellular degradative systems are able to recognize the misfolded proteins and provide their degradation and clearance, through the ubiquitin-proteasome…Find out more