The generation of cell lines overexpressing particular proteins on which drug discovery programs are often based, is necessary for assay development in screening campaigns using initial target-based identification of hit compounds. Recombinant stable cell lines are also used for toxicity studies and general in vitro testing.
Methods to be used and the cell background for the generation of a cell line could depend on several factors: i) the complexity of protein (e.g. dimensions, carrying antibiotic resistance, presence of tags etc.)…Find out more