X-ray Crystallography
We offer crystallography services from protein production and crystallization to data collection
and structure determination, with streamlined protocols and guaranteed reproducibility.
State-of-the-art crystallography
equipment
- Gryphon nano dispensing crystallization robot
- Crystal imaging microscopes and cameras
- Specialized tools for crystal handling
Multiple contracting
and reporting options
International team of highly skilled scientists with proven scientific record
- Solved over 170 high-resolution structures
- Delivered over 60 peer-reviewed
publications
Access to crystallographic software
(XDS, CCP4) and high-speed
processing computers
Secured regular access to synchrotron measurements at top European facilities
- Pre-scheduled access
- On-demand access
Protein crystallography and high-resolution structure determination
Broad range of targets
- Protein-protein
- Protein-small molecule
- Protein-peptide
- Protein-nucleic acid
- Various protein families
Over 1500 crystallization
conditions from commercial
crystallization screens
- With possibility to obtain
additional/custom screens
Various methods
- Molecular Replacement
- Experimental Phasing
(metals, halogens,
sulphur, SeMet)
Screening of 1600+ crystallization
conditions per day
- Validation of published crystallization conditions
- Identification of novel crystallization conditions
- Diffraction tests
Multiple set-ups
- Vapour diffusion: hanging/sitting drops
- Drop sizes from 80 nL to 4 µL
- Crystal seeding
- Crystal soaking
Extensive hit
optimization
- Grid screening
- Multiple seeding approaches
- High-quality reagents
Large-scale, high-quality protein production
Tailored recombinant
proteins
- Custom construct design to
maximize quality and propensity
for crystallization - Multiple expression systems
(bacterial, insect, mammalian cells) - SeMet incorporation
- Co-expression and co-purification
with small molecules
High homogeneity, purity
and stability of produced proteins
- Confirmed by Standard Quality Control
- Verified batch-to-batch consistency
- Crystallization-grade quality
From tens to hundreds
of milligrams of purified protein
Structure-based drug design
Crystallography offers efficient way to screen both lead candidate molecules and larger libraries of potential hits
offering valuable information about place and mode of interaction.
Various complex preparation strategies
- Co-crystallization
- Development of soakable systems
- Co-expression/co-purification
- Robust structure determination
Integrated services
- Close collaboration with Medicinal and Computational Chemistry, Biology, Biophysics
- Biophysical, biochemical and enzymatic assays (e.g. DSF, ITC, SPR)
- Evaluation of target protein structure
Hit-to-lead development, lead optimization
Fragment-based drug design
Our services are tailored to your needs
Understanding specific features
of your project
- Overcoming bottle-necks in availability of your internal resources
- Flexible entry and exit points
- FTE and FFS collaboration models
Flexibility in deliverable and reporting formats
- Detailed reporting
- Option to transfer raw diffraction data
Standalone or combined services
Involvement in decision making
Integrated services:
support from other Selvita departments