Standard Quality Control

• Purity by SDS-PAGE followed by CBB staining – densitometric analysis
• Concentration determined spectrophotometrically using the calculated mass and extinction coefficient or A₂₈₀ = 1 for 0.1% protein solution, alternatively Bradford, BCA or SDS-PAGE densitometric method
• The homogeneity and oligomerization status assessed by the HPLC-SEC
• Identity verified by N-terminal sequencing (Edman method) or LC-MS/MS analysis, Peptide mapping or intact mass
• Overall quality/folding assessed by the nanoDSF or DSF (TSA)
• Protein contamination with nucleic acids assessed by the ratio of A₂₆₀/A₂₈₀
• Determination of purity by RP-HPLC, Activity/Sterility testing, Determination of endotoxins levels, Determination of biotinylation level