Immuno-inflammation

Selvita offers assays on whole blood, peripheral blood mononuclear cells, isolated blood cells (T and B lymphocytes, monocytes/macrophages, dendritic cells, neutrophils), immune cells isolated from tissues (e.g. tonsils, lamina propria) as well as other types of primary cells and cell lines. The assays are done using cells from healthy donors or patients. Our portfolio of in vitro assays covers various types of functional responses related to immunological response activation with multiple readouts and endpoints.

Assay specifics:

• Various triggers
• Readouts translatable to in vivo models and clinics
• Tailor-made assays based on client’s needs

The assays are designed to monitor the effect of compounds on various functions of the cells:

• Cell viability, proliferation and differentiation
• Cell migration
• Phagocytosis
• Cell surface markers expression
• Activation of signaling pathways
• Inflammatory mediator production

In vivo inflammatory models

With our vast collection of inflammatory models and read-outs, Selvita supports Your drug discovery projects targeting general and local inflammatory diseases.

Acute general inflammation

• LPS-induced inflammation (systemic route)
• LPS-induced endotoxemia in rats
• Septic shock in mice
• NLRP3 inflammasome activation in mice
• Peritonitis in mice (Zymosane A and Thioglycollate)
• Anti-CD3-induced acute inflammation in mice
• Air pouch
• Ear oedema in mice and rats
• PMA-induced ear oedema in mice
• Croton oil-induced ear oedema in rats
• Cantharidin-induced ear oedema in mice

Respiratory inflammation

COPD

• Acute (3-day) tobacco smoke induced lung inflammation in mice
• Subacute (11-day) tobacco smoke induced lung inflammation in mice
• LPS induced pulmonary inflammation (intranasal challenge)
• Acute COPD exacerbation induced by Influenza A virus Puerto Rico/8/1934(H1N1)

Asthma

• Ovalbumin induced allergic asthma in mice
• HDM induced allergic asthma in mice
• HDM/CFA severe neutrophilic asthma in mice
• IL-13 triggered pSTAT6 formation in mouse lungs
• Asthma exacerbation induced by Influenza A virus Puerto Rico/8/1934(H1N1)

Read-outs for respiratory inflammation models:

• Total and differential cell count in BALF
• Lung function (resistance and compliance)
• Inflammatory mediators in BALF
• Histopathology
• IHC/Digital image analysis
• IF/ISH

Gastrointestinal portfolio (functional and disease models)

• Acute and chronic DSS induced colitis in mice/rats
• TNBS induced colitis in rats
• Oxazolone induced colitis in rats
• CD4+CD25-CD62L+ T cell transfer model of chronic colitis in C.B-17 SCID mice
• GI transit in mice and rats
• GI permeability and motility evaluation in mice and rats
• Gastric emptying and acid secretion
• Intestinal loops ligation in mice and rats
• Intestinal mass growth
• Food allergy model in mice
• Oral glucose tolerance test (OGTT) in mice
• MTX induced mucositis in rats

Read-outs for gastrointestinal portfolio:

• Disease activity score (DAI)
• Colon length (stretching)
• Histopathology
• IHC/Digital image analysis
• IF/ISH
• Inflammatory mediators in colon tissue

Autoimmune diseases

• Collagen type-II induced arthritis in mice and rats
• Collagen antibody-induced arthritis (CAIA)
• Systemic lupus erythematosus (SLE) in mice
• TRP2-180 induced vitiligo

Read-outs:

• Clinical scoring
• Paw swelling
• Hematology
• Histopathology
• IHC
• Serum biomarkers
• Inflammatory mediators

Skin inflammation

• Keyhole limpet hemocyanin (KLH)-induced delayed type hypersensitivity (DTH) in mice and rats (acute)
• Oxazolone-induced DTH in mice (acute and chronic)
• Urushiol-induced DTH in mice (acute)
• Wound healing model in mice and rats
• Impaired wound healing models (hyperglycemic rats; db/db mice)
• IMQ-induced psoriasis in mice

For more information on our capabilities in the area of inflammatory diseases please contact us.