Selvita offers assays on primary cells and lung tissue focusing on myofibroblast activation, extracellular matrix production, tissue remodelling and macrophage polarisation.
The in vitro assays for evaluation of prospective anti-fibrotic drugs include:
- Fibroblast to myofibroblast differentiation assay
- Epithelial to mesenchymal transition assay
- Proliferation and viability of primary human fibroblast and epithelial cells
- M1 and M2 polarisation of monocytes/macrophages
We also offer ex vivo assays in tissue explants and precision-cut slices e.g.:
- Lung tissue from mice with bleomycin-induced fibrosis
- Human lung tissue treated with different pro-fibrotic factors
Assay read-outs:
- Gene expression (qPCR)
- Protein expression (high content imaging, western analysis, ELISA, MSD, Luminex, flow cytometry)
- Cell proliferation, metabolic activity, apoptosis, membrane integrity
In vivo models of fibrosis
Bleomycin-induced pulmonary fibrosis (mice)
Read-outs:
- Lung weight
- Histopathological analysis
- Ashcroft score
- Collagen content by quantitative digital image analysis
- aSMA by quantitative digital image analysis
- IHC/IF/ISH
- Digital image analysis
- Lung function measurements (FEV, FVC)
- Total and differential cell counts in bronchoalveolar lavage fluid
- Gene expression
- Hydroxyproline concentration in lung tissue homogenates
- LPA species in serum and bronchoalveolar lavage fluid
CCl4-induced liver fibrosis (mice)
Read-outs:
- Collagen deposition by quantitative digital image analysis
- Gene expression
CDHFD-induced NASH and liver fibrosis (mice)
Read-outs:
- NAS-score
- Collagen deposition/aSMA/macrophage accumulation by quantitative digital image analysis
- IHC/IF/ISH
- Digital image analysis
- Gene expression
- Liver enzymes
Unilateral ureteral obstruction (UUO) model of renal fibrosis (mice)
Read-outs:
- Gene expression in kidneys
Folic acid-induced renal fibrosis (mice)
Read-outs:
- Collagen deposition by quantitative digital image analysis
For more information about our fibrosis models please contact us.